[3] Modified Russell–Movat staining highlights numerous tissue components in histological slides.
It is obtained by a mix of five stains: alcian blue, Verhoeff hematoxylin and crocein scarlet combined with acidic fuchsine and saffron.
The Verhoeff hematoxylin has a high affinity for nuclei and elastin fibers, negatively charged.
The combination of crocein scarlet with acidic fuchsine stains acidophilic tissue components in red.
Then, collagen and reticulin fibers are unstained by a reaction with phosphotungstic acid and stained in yellow by saffron.