The route gains a center left-turn lane and continues into business areas on the outskirts of town, coming to a ramp that provides access to I-70/I-76 (Pennsylvania Turnpike).

After this, PA 601 briefly becomes a divided highway before regaining the center left-turn lane and coming to a bridge over the Pennsylvania Turnpike.

The route becomes Front Street and runs through the residential community of Jenners prior to coming to an intersection with US 30 in Ferrellton.

[2][3] Past this, the road curves east through a mix of farms, woods, and homes before heading into woodland and turning to the north.

The route runs along the west bank of the Stonycreek River as it turns east and crosses PA 403 in rural residential areas.

Mainline PA 601 experiences heavy truck volume due to its access to the Pennsylvania Turnpike at Exit 110.