The 2021 Copa do Brasil second round was the second round of the 2021 Copa do Brasil football competition.
[1] A total of 40 teams competed in the second round to decide 20 places in the third round of the 2021 Copa do Brasil.
[2] In the second round, each tie was played on a single-legged basis.
If the score was level, the match would go straight to the penalty shoot-out to determine the winners.
[2] Host teams were settled in the first-round draw.