Independiente del Valle won their first title in the competition, defeating 9 de Octubre in the final by a 3–1 score.
[2] LDU Quito were the defending champions, having won the previous edition of the competition in 2019, but were knocked out by Imbabura in the round of 16.
The 16 winners advanced to the round of 32, where they were drawn against the 16 Serie A teams in single-legged ties with the lower-tier side hosting the match.
The top two teams in the semi-final group advanced to the final.
[5] In case the champions qualified for the Copa Libertadores through their league or international performance, the berth would be transferred to the runners-up, the semi-finalists (provided they were Serie A teams for the following season), or the next best team in the Ecuadorian Serie A not yet qualified for that competition.