U.S. Route 29 or U.S. Highway 29 (US 29) is a north–south United States Numbered Highway that runs for 1,043.3 miles (1,679.0 km) from Pensacola, Florida, to Ellicott City, Maryland, just west of Baltimore, Maryland, in the Eastern United States, connecting the Florida Panhandle to the Washington–Baltimore combined statistical area.

From Auburn, Alabama, to Greensboro, I-85 runs parallel to US 29, which serves primarily as a local route along that stretch.

Today, US 29/SR 15 serves primarily to connect numerous smaller towns and cities in the southwest, south-central, and eastern parts of Alabama, notably passing near Troy, Tuskegee, and Auburn universities in the east.

Large crowds gathered along US 29 on this day in April 1945 to pay their final respects to the deceased president.

Unfortunately, for those who waited along the highway, they missed seeing the president's body being transported back to Washington DC on a train that ran on nearby tracks.

In South Carolina, US 29 maintains a northeasterly routing, passing through Anderson, Greenville, and Spartanburg.

In North Carolina, US 29 connects the cities of Gastonia, Charlotte, Concord, Salisbury, High Point, Greensboro, and Reidsville.

After leaving the Charlotte metropolitan area, the road stays parallel with I-85 and concurrent with US 70 and serves as a secondary highway for cities along the Interstate.

It stays parallel with I-85 for another 40 miles (64 km) before branching off in Greensboro and heading north towards Reidsville and then to Virginia towards Danville.

US 29 connects the historic small cities and large towns of west-central Virginia, including Danville, Lynchburg, Charlottesville, Culpeper, and Warrenton, with Fairfax, Falls Church, Arlington, and Washington DC to the northeast and with North Carolina to the southwest.

On either side of the road through the battlefield, split rail fences define property borders.

[2] The freeway stretch of US 29 traveling southwest from Greensboro to Lexington, North Carolina, is currently also signed as I-85 Business (I-85 Bus.)

This plan, according to the state, will simplify overhead signage on the freeway and eliminate the confusion between I-85 and I-85 Bus.