The von Kossa histological stain is used to quantify mineralization in cell culture and histological sections.
This is a staining method to illustrates mineralization such as calcium and potassium in tissues.
[1][2][3][4][5] It is a precipitation reaction in which silver ions react with phosphate in the presence of acidic material.
[6] Photochemical degradation of silver phosphate to silver happens under light illumination.
Additional methods need to be employed to confirm the presence of calcium, such as Alizarin Red S.[citation needed]