The 2010 Open Castilla y León was a professional tennis tournament played on outdoor hard courts.

It was the twenty fifth edition of the tournament which was part of the Tretorn SERIE+ of the 2010 ATP Challenger Tour.

It took place in Segovia, Spain between 2 and 8 August 2010.

The following players received wildcards into the singles main draw: The following players received entry from the qualifying draw: Daniel Gimeno-Traver def.

Adrian Mannarino, 6–4, 7–6(2) Thiago Alves / Franco Ferreiro def.