Finally, in the round of 16, the eight third stage winners were joined by the four Copa Libertadores qualifiers (Junior, América de Cali, Deportes Tolima, and Independiente Medellín), as well as the four Copa Sudamericana qualifiers (Deportivo Cali, Atlético Nacional, Millonarios, and Deportivo Pasto), which entered the competition at this point.
[2] Due to the suspension of the competition caused by the COVID-19 pandemic, on 9 September 2020 DIMAYOR announced that, starting from the round of 16, all subsequent stages up to the final would be played as single-legged ties instead of double-legged ones as originally planned.
[3] The schedule of the competition was as follows, with most rounds rescheduled due to the COVID-19 pandemic:[4][5] The first stage was played by the 16 Categoría Primera B clubs, eight of which were seeded in the ties according to their placement in the 2019 season aggregate table.
The two promoted clubs from the Categoría Primera B (Deportivo Pereira and Boyacá Chicó) were the last two seeded teams, with Deportivo Pereira taking the eleventh position and Boyacá Chicó the twelfth position.
In case of a tie, extra time was not applied and the winner was decided in a penalty shoot-out.