Phenol red

Phenol red (also known as phenolsulfonphthalein or PSP) is a pH indicator frequently used in cell biology laboratories.

If the pH is increased (pKa = 1.2), the proton from the ketone group is lost, resulting in the yellow, negatively charged ion denoted as HPS−.

At still higher pH (pKa = 7.7), the phenol's hydroxy group loses its proton, resulting in the red ion denoted as PS2−.

Phenol red was used by Leonard Rowntree and John Geraghty in the phenolsulfonphthalein test to estimate the overall blood flow through the kidney in 1911.

A small amount of phenol red added to this growth medium will have a pink-red color under normal conditions.

In addition, the waste products produced by the mammalian cells themselves will slowly decrease the pH, gradually turning the solution orange and then yellow.

[12] Phenol red, sometimes labelled with a different name, such as "Guardex Solution #2", is used as a pH indicator in home swimming pool test kits.

Phenol red
Phenol red
Phenol red
Phenol red
Phenol red, 40 μM: colors in cell culture medium at a pH range from 6.0 to 8.0.
A commercial test kit for swimming pools with phenol red and an orthotolidine indicator solution