Suppressor mutation

[1] Suppressor mutations are useful for identifying new genetic sites which affect a biological process of interest.

[1] In addition to the reading frame, Crick also used suppressor mutations to determine codon size.

Several proteins of biochemical, signal transduction, and gene expression pathways have been identified using this approach.

These intermediate strains are subjected to bottlenecking and thus making it difficult for the alleles to be reverted prior to Intergenic suppressions.

If, upon infection, an amber mutant defective in a gene encoding a needed structural component of phage T4 is weakly suppressed (in an E. coli host containing a specific altered tRNA – see nonsense suppressor), it will produce a reduced number of the needed structural component.

[4] It was found that the reason the second amber mutation could suppress the first one is that the two numerically reduced structural proteins would now be in balance.