Taekwondo athlete Miguel Trejos and boxer Yeni Arias were the country's flagbearers during the opening ceremony.

[1] Meanwhile, squash athlete Miguel Ángel Rodríguez and roller speed skater Fabriana Arias were the country's flagbearers during the closing ceremony.

As Barranquilla was supposed to be host the 2027 Pan American Games, a Colombian segment was performed at the closing ceremony.

[5][6] Colombia qualified a men's team (of 24 athletes) by winning the 2021 Junior Pan American Games.

Colombia qualified a women's team (of 4 athletes) by winning the 2021 Junior Pan American Games.

[8] Colombia qualified a full team of two men and two women through the 2022 South American Games held in Asunción, Paraguay.

[14] Colombia qualified one female cyclist in Mountain biking after winning the event in the 2021 Junior Pan American Games.

[24] Colombia qualified a team of ten gymnasts in artistic (five men and five women) at the 2023 Pan American Championships.

[42] Colombia qualified one male swimmer to the Open Water event during the 2022 South American Games.

Colombia also qualified 3 swimmers (Two men and one women) after by winning respective events in the 2021 Junior Pan American Games.

[44] Colombia has qualified a male athlete at a Kyorugi event, by virtue of his title in the 2021 Junior Pan American Games.

[45] Colombia also qualified 6 athletes (three men and three women) during the Pan American Games Qualification Tournament.

Colombia also qualified five weightlifters (Four men and two women) after winning the event in the 2021 Junior Pan American Games.