Ecuador competed at the 2023 Pan American Games in Santiago, Chile from October 20 to November 5, 2023.

On 2 October 2023, the Ecuadorian National Olympic Committee officially named the team of 167 athletes competing in thirty one sports.

[1] Athlete Brian Pintado and weightlifter Angie Palacios were named as the country's flagbearers during the opening ceremony.

Ecuador qualified a team of two men through the 2022 South American Games held in Asuncion, Paraguay.

[14] Ecuador qualified four cyclists (two men and two women) in BMX race through the UCI World Rankings.

[16] Ecuador qualified one female fencer through the 2022 Pan American Fencing Championships in Ascuncion, Paraguay.

Ecuador qualified four gymnasts in artistic (two men and two women) at the 2023 Pan American Championships.

[26] Ecuador qualified a total of nine shooters (six men and three women) after the 2022 Americas Shooting Championships and the 2022 South American Games.

[27][28][29][30][31][32] Ecuador qualified a team of 6 climbers (three men and three women) by virtue of their IFSC world rankings.

Ecuador qualified a team of three female athletes through the 2023 Pan American Squash Championships.

Ecuador qualified 8 athletes (four men and four women) during the Pan American Games Qualification Tournament.